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- FceR1 alpha Antibodies
Invitrogen
View all (56) FceR1 alpha antibodies
Cite FceR1 alpha Monoclonal Antibody (AER-37 (CRA1)), eBioscience™
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FceR1 alpha Antibody (14-5899-82) in Flow
Staining of normal human peripheral blood cells with Mouse IgG2b kappa Isotype Control Purified (Product # 14-4732-82) (green histogram) or Anti-Human Fc epsilon Receptor I a (FcepsilonR1) Purified (blue histogram) followed by Anti-Mouse IgG Biotin (Product # 13-4013-85) and Streptavidin PE (Product # 12-4317-87). Cells in the lymphocyte gate were used for analysis. {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
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Product Details
14-5899-82
Applications
Tested Dilution
Publications
Immunohistochemistry (IHC)
-
View 1 publication1 publication
Immunocytochemistry(ICC/IF)
-
View 1 publication1 publication
Flow Cytometry(Flow)
1 µg/test
View 4 publications4 publications
Neutralization(Neu)
-
View 1 publication1 publication
Functional Assay(FN)
-
View 1 publication1 publication
Product Specifications
Species Reactivity
Human
Published species
Human
Host/Isotype
Mouse/ IgG2b, kappa
Class
Monoclonal
Type
Antibody
Clone
AER-37 (CRA1)
Conjugate
Unconjugated
- APC
- Biotin
- Brilliant Violet 786
- eFluor 450
- FITC
- Functional Grade
- NovaFluor Blue 610-70S
- NovaFluor Blue 660-120S
- NovaFluor Yellow 570
- NovaFluor Yellow 610
- NovaFluor Yellow 660
- PE
- PE-Cyanine7
- PE-eFluor 610
- PerCP-eFluor 710
- Super Bright 436
- Super Bright 600
- Super Bright 780
Form
Liquid
Concentration
0.5 mg/mL
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.2
Contains
0.09% sodium azide
Storage conditions
4° C
Shipping conditions
Wet ice
RRID
AB_467710
Product Specific Information
Description: The AER-37 monoclonal antibody reacts with the FcεRIalpha subunit, an IgE-binding subunit lacking signal-transducing ability. FcεRIalpha is expressed on mast and basophil cells and is upregulated by the presence of IgE. FcεRIalpha forms a tetrameric complex with one beta and two gamma subunits. The beta and gamma subunits possess immunoreceptor tyrosine-based activation motifs (ITAM). The FcεRI complex plays an important role in triggering IgE-mediated allergic reactions.
Applications Reported: The AER-37 (CRA1) antibody has been reported for use in flow cytometric analysis. It has been reported to not block IgE binding.
Applications Tested: The AER-37 (CRA1) antibody has been tested by flow cytometric analysis of peripheral blood cells. This can be used at less than or equal to 1 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
Purity: Greater than 90%, as determined by SDS-PAGE.
Aggregation: Less than 10%, as determined by HPLC.
Filtration: 0.2 µm post-manufacturing filtered.
Target Information
FceR1 (Fc epsilonR1 alpha) is a subunit of the high affinity receptor of IgE. Fc epsilonR1 alpha is a tetrameric complex consisting of one alpha, one ß and two additional subunits. The latter two are required for signal transduction activity. The Fc epsilonR1 complex plays an important role in triggering allergic responses, and the immunoglobulin epsilon receptor (IgE receptor) is the initiator of the allergic response. When two or more high-affinity IgE receptors are brought together by allergen-bound IgE molecules, mediators such as histamine, which are responsible for allergy symptoms, are released. The IgE receptor couples allergen and mast cells to initiate the inflammatory responses that are characteristic of disorders such as hay fever and asthma. The release of histamine and proteases leads to the synthesis of prostaglandins and leukotrienes-potent effectors of the hypersensitivity response.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases:fc epsilon r1; Fc epsilon receptor Ia; Fc epsilon RI alpha-chain; Fc fragment of IgE, high affinity I, receptor for; alpha polypeptide; Fc IgE receptor, alpha polypeptide; Fc-epsilon RI-alpha; FcERI; high affinity immunoglobulin epsilon receptor alpha-subunit; High affinity immunoglobulin epsilon receptor subunit alpha; IgE Fc receptor subunit alpha; immunoglobulin E receptor, high-affinity, of mast cells, alpha polypeptide; RP11-550P17.3
UniProt ID:(Human) P12319
Entrez Gene ID:(Human) 2205
Molecular Function: 
immunoglobulin receptor superfamily
Function(s)
positive regulation of type I hypersensitivityserotonin secretionactivation of JUN kinase activityleukotriene biosynthetic processFc-epsilon receptor signaling pathwaypositive regulation of mast cell degranulationpositive regulation of interleukin-3 biosynthetic processpositive regulation of granulocyte macrophage colony-stimulating factor biosynthetic processpositive regulation of peptidyl-tyrosine phosphorylationpositive regulation of calcium-mediated signaling
Process(es)
IgE receptor activityIgE binding
It has to be done as per old AB suggested Products section.
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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