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- CD40 Antibodies
Leinco Technologies
View all (182) CD40 antibodies
Cite CD40 Monoclonal Antibody (G28.5), Functional Grade, In Vivo PLATINUM™
Product Details
C8205-100MG
Applications
Tested Dilution
Publications
Flow Cytometry(Flow)
Assay-dependent
-
Functional Assay(FN)
Assay-dependent
-
Product Specifications
Species Reactivity
Human
Host/Isotype
Mouse/ IgG1, kappa
Class
Monoclonal
Type
Antibody
Clone
G28.5
Immunogen
Human tonsillar lymphocytes
Form
Liquid
Concentration
≥5.0 mg/mL
Purification
Protein A/G
Storage buffer
PBS, pH 7.2-7.4
Contains
no preservative
Storage conditions
4°C short term, -80°C long term
Shipping conditions
Wet ice
Product Specific Information
Specificity: Clone G28.5 recognizes an epitope on human CD40.
Endotoxin Level: <0.5 EU/mg as determined by the LAL method.
Purity: ≥98% monomer by analytical SEC and >95% by SDS Page.
Preparation: Functional grade preclinical antibodies are manufactured in an animal free facility using in vitro cell culture techniques and are purified by a multi-step process including the use of protein A or G to assure extremely low levels of endotoxins, leachable protein A or aggregates.
Pathogen Testing: To protect mouse colonies from infection by pathogens and to assure that experimental preclinical data is not affected by such pathogens, all of Leinco Purified Functional PLATINUM™ antibodies are tested and guaranteed to be negative for all pathogens in the IDEXX IMPACT I Mouse Profile.
Storage: Functional grade preclinical antibodies may be stored sterile as received at 2-8C for up to one month. For longer term storage, aseptically aliquot in working volumes without diluting and store at -80C. Avoid Repeated Freeze Thaw Cycles.
Target Information
The CD40 antigen is a single chain glycoprotein that is known to be a member of the tumor necrosis factor/nerve growth factor superfamily and shows a significant hom*ology to the Hodgkin's disease associated antigen, CD30. CD40 is present on all B cells except plasma cells and is also found on some epithelial cells, carcinomas and lymphoid dendritic cells. CD40 has been found to be essential in mediating a broad variety of immune and inflammatory responses including T cell-dependent immunoglobulin class switching, memory B cell development, and germinal center formation. AT-hook transcription factor AKNA is reported to coordinately regulate the expression of CD40, which may be important for hom*otypic cell interactions. Adaptor protein TNFR2 interacts with CD40 and serves as a mediator of the signal transduction. The interaction of CD40 and its ligand is found to be necessary for amyloid-beta-induced microglial activation, and thus is thought to be an early event in Alzheimer disease pathogenesis. Two alternatively spliced transcript variants of CD40 encoding distinct isoforms have been reported. Diseases associated with CD40 dysfunction include Type 3 Hyper-Igm immunodeficiency and CD40 ligand deficiency.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases:B cell surface antigen CD40; B cell-associated molecule; B-cell surface antigen CD40; Bp50; CD antigen CD40; CD40; CD40 molecule, TNF receptor superfamily member 5; CD40L receptor; CDw40; Immunoglobulin M; ImmunoglobulinM; MGC9013; sCD40; soluble CD40; Tumor necrosis factor receptor superfamily member 5
UniProt ID:(Human) P25942
Entrez Gene ID:(Human) 958
Molecular Function: 
transmembrane signal receptor
Function(s)
positive regulation of protein phosphorylationimmune response-regulating cell surface receptor signaling pathwayprotein complex assemblycellular calcium ion homeostasisinflammatory responseimmune responsemulticellular organism developmentplatelet activationpositive regulation of B cell proliferationresponse to lipopolysaccharidepositive regulation of interleukin-12 productiontumor necrosis factor-mediated signaling pathwayB cell proliferationregulation of cell proliferationpositive regulation of tyrosine phosphorylation of Stat1 proteindefense response to protozoanpositive regulation of I-kappaB kinase/NF-kappaB signalingpositive regulation of MAP kinase activityprotein kinase B signalingpositive regulation of GTPase activitypositive regulation of transcription from RNA polymerase II promoterpositive regulation of isotype switching to IgG isotypesregulation of immune responseregulation of immunoglobulin secretionpositive regulation of NF-kappaB transcription factor activitydefense response to viruscellular response to lipopolysaccharidecellular response to mechanical stimuluspositive regulation of protein kinase C signalingapoptotic signaling pathwaypositive regulation of endothelial cell apoptotic process
Process(es)
antigen bindingsignal transducer activityreceptor activitytumor necrosis factor-activated receptor activityprotein bindingenzyme bindingubiquitin protein ligase binding
It has to be done as per old AB suggested Products section.
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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